I was reading a paper and found that the authors calculated a score of a gene list for each sample separately based on the following steps: 1) mean center, 2) average over genes, 3) Z-score. The description seems a little bit confusing to me and I want a specific example.
Here is a dataframe. How can I calculate the signature score for these two samples based on the method described above? (R code is welcome)
Sample1 Sample2 Gene_A 0.3019117 0.3649211 Gene_B 0.2861431 0.3072168 Gene_C 0.3794475 0.6505417 Gene_D 0.2794465 0.3906110 Gene_E 0.3334156 0.5845917 Gene_F 0.3513268 0.6560779